Ristocetin Cofactor Assay

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INTENDED USE

The CHRONO-LOG® Ristocetin Cofactor Assay is for use in the quantitative determination of Factor VIII Ristocetin Cofactor activ­ity in citrated plasma.

SUMMARY

Ristocetin Cofactor Activity in plasma may be determined by the agglutination of a standardized suspension of platelets in the presence of von Willebrand factor using the antibiotic Ristoce­tin.

1. The Ristocetin Cofactor Activity is the in-vitro activity of the von Willebrand factor which is responsible for the agglutination of platelets in the presence of Ristocetin. Although the platelets play a passive role in such agglutination there is an absolute require­ment that the Ristocetin-dependent receptor be intact.

2. von Wil­lebrand disease is associated with a decrease in von Willebrand factor or Ristocetin Cofactor activity and it is generally accepted that the Ristocetin Cofactor activity is the most useful in vitro assay for the diagnosis of von Willebrand disease.

3. Four Levels of Ristocetin Cofactor activity are determined by the ability of the test plasma and Ristocetin to induce aggregation in a standard­ized platelet suspension.

PRINCIPLE

Following reconstitution, the lyophilized platelets are treated with Ristocetin in the presence of dilutions of a normal standardized human plasma with known amounts of Ristocetin Cofactor activ­ity. A standard curve is prepared, a patient plasma is then used as a source of Ristocetin Cofactor activity in the presence of Ristocetin and reconstituted platelets from which an aggregation pattern is determined and the Ristocetin Cofactor activity is inter­polated from the standard curve.[/woodmart_text_block][vc_single_image image=”1336″ img_size=”full” alignment=”center” parallax_scroll=”no” woodmart_inline=”no”][/vc_column][/vc_row][vc_row el_class=”sec-2-pd” woodmart_css_id=”6904ff4c15ee0″ responsive_spacing=”eyJwYXJhbV90eXBlIjoid29vZG1hcnRfcmVzcG9uc2l2ZV9zcGFjaW5nIiwic2VsZWN0b3JfaWQiOiI2OTA0ZmY0YzE1ZWUwIiwic2hvcnRjb2RlIjoidmNfcm93IiwiZGF0YSI6eyJ0YWJsZXQiOnt9LCJtb2JpbGUiOnt9fX0=” mobile_bg_img_hidden=”no” tablet_bg_img_hidden=”no” woodmart_parallax=”0″ woodmart_gradient_switch=”no” woodmart_box_shadow=”no” wd_z_index=”no” woodmart_disable_overflow=”0″ row_reverse_mobile=”0″ row_reverse_tablet=”0″ css=”.vc_custom_1761935197577{margin-top: 30px !important;}”][vc_column width=”1/3″]

P/N 299 Ristocetin Cofactor Assay Kit

P/N 299-1 Ristocetin

P/N 299-2 Lyophilized Human Platelets, TBS

P/N 299-3 VW Reference Plasma Normal 1mL

P/N 299-4 VW Reference Plasma Deficient 0.5mL

P/N 299-5 Tris Buffered Saline 12mL

P/N 299-6 Hemostasis Reference Plasma Normal 1mL

P/N 299-7 Hemostasis Reference Plasma Abnormal 1mL